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We present a phylogenetic dating of asterids, based on a taxon tree representing all major groups and orders and 83 of the families of asterids, with an underlying data set comprising six chloroplast DNA markers totaling positions. Phylogenetic dating was done with semiparametric rate smoothing by penalized likelihood. Confidence intervals were calculated by bootstrapping. Six reference fossils were used for calibration.
To explore the effects of various sources of error, we repeated the analyses with alternative dating methods nonparametric rate smoothing and the Langley-Fitch clock-based method , alternative tree topologies, reduced taxon sampling 22 of the taxa deleted , partitioning the data into three genes and three noncoding regions, and calibrating with single reference fossils.
The analyses with alternative topologies, reduced taxon sampling, and coding versus noncoding sequences all yielded small or in some cases no deviations. The choice of method influenced the age estimates of a few nodes considerably. Calibration with reference fossils is a critical issue, and use of single reference fossils yielded different results depending on the fossil.
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